英语翻译摘要:硝酸盐在植物体内积累过多已成为影响蔬菜品质并影响人类健康的重要因素,硝酸还原酶是硝酸盐代谢中的关键酶,提高其活性有利于硝酸盐的降解.本文采用离体法测定不同条
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英语翻译摘要:硝酸盐在植物体内积累过多已成为影响蔬菜品质并影响人类健康的重要因素,硝酸还原酶是硝酸盐代谢中的关键酶,提高其活性有利于硝酸盐的降解.本文采用离体法测定不同条
英语翻译
摘要:硝酸盐在植物体内积累过多已成为影响蔬菜品质并影响人类健康的重要因素,硝酸还原酶是硝酸盐代谢中的关键酶,提高其活性有利于硝酸盐的降解.本文采用离体法测定不同条件对于茎瘤芥硝酸还原酶活性的影响.茎瘤芥硝酸还原酶经过不同的pH、不同的温度、不同的时间、不同的硝酸钾浓度以及硝酸还原酶不同的浓度的处理表明:茎瘤芥硝酸还原酶的最适pH为6.8;最适温度为25℃;最适底物硝酸钾浓度为mol/L,在茎瘤芥硝酸还原酶相对浓度足够大时,随着底物浓度增加,茎瘤芥硝酸还原酶的活性逐渐上升.反应条件超过或者达不到最适条件时酶活性都会受到抑制.同时,在底物硝酸钾浓度足够大时,酶液相对浓度较低的情况下,逐渐增加酶液相对浓度,反应速度随着硝酸还原酶相对浓度的增加而增加,酶反应速率也随之增加.
关键词:硝酸还原酶 pH 温度 时间 硝酸钾浓度 激活剂 抑制剂
英语翻译摘要:硝酸盐在植物体内积累过多已成为影响蔬菜品质并影响人类健康的重要因素,硝酸还原酶是硝酸盐代谢中的关键酶,提高其活性有利于硝酸盐的降解.本文采用离体法测定不同条
The nitrate had accumulated the healthy key factor already have become the quality affecting vegetable,and affecting human being within the plant body,hydrogen nitrate reductase key enzyme in being that the nitrate metabolizes ,nitrate beneficial to improving whose activity degradation.The main body of a book is adopt to follow different condition of admeasurement in vitro to active effect of stem tumour mustard hydrogen nitrate reductase.Different course the different pH ,the different temperature ,different time ,different potassium nitrate thickness and hydrogen nitrate reductase thickness of tumour mustard hydrogen nitrate reductase treatment indicates a stem:The most suitable stem tumour mustard hydrogen nitrate reductase pH is 6.8; The most suitable temperature is 25 C; The most suitable bottom thing potassium nitrate thickness is mol/L ,thickness is relatively sufficient in stem tumour mustard hydrogen nitrate reductase when big ,stem tumour mustard hydrogen nitrate reductase activity rises gradually with the fact that bottom thing thickness increases by.Reaction condition exceeds or the enzymatic activity city accepts when being unable to reach the most suitable condition restraint.Simultaneous ,be enough in bottom thing potassium nitrate thickness when big,under lower condition of relative enzyme liquid thickness,the relative thickness increasing enzyme liquid gradually ,relative with hydrogen nitrate reductase reaction speed thickness increasing by but increase by,the enzyme reaction rate increases by also with that.Keywords:Hydrogen nitrate reductase pH temperature time potassium nitrate thickness activator depressor