化学论文翻译Until now, no article reported the identification and quantification ofP. aeruginosa using MSPQC.In this article, a new acetamide broth was developed for identificationand quantification of P. aeruginosa by MSPQC. It was based on th

化学论文翻译Until now, no article reported the identification and quantification ofP. aeruginosa using MSPQC.In this article, a new acetamide broth was developed for identificationand quantification of P. aeruginosa by MSPQC. It was based on th
化学论文翻译
Until now, no article reported the identification and quantification of
P. aeruginosa using MSPQC.
In this article, a new acetamide broth was developed for identification
and quantification of P. aeruginosa by MSPQC. It was based on the fact
that P. aeruginosa can grow well in a medium using acetamide as nitrogen
and carbon source (Noel and Holt 1984; Smith and Dayton 1972). Very
few bacteria can do this. During the process of growing P. aeruginosa,
the conductivity of the medium changed and was automatically monitored
by MSPQC. Frequency detection time (FDT), which is defined as time at
which oscillating frequency shift of MSPQC changed rapidly, has a linear
relationship with the logarithm of concentration. It is a parameter to
quantitatively determine P. aeruginosa by MSPQC sensor.
EXPERIMENTAL
Bacterial Isolation
Pseudomonas aeruginosa (ATCC 9027), Staphylococcus aureus (ATCC
12600), Acinetobacter baumannii (ATCC 19606), Escherichia coli (ATCC
11775), and Pseudomonas maltophilia (ATCC 13637) were obtained
from Third Xiangya Hospital of Central South University, China.
All the species were inoculated on blood plate (purchased from
Guangdong Huankai Microbial Science and Technology Co. Ltd.) at
37\2C for 18 h, respectively. Then, three loops of each species in pure
culture were transferred into a 100-ml sterilized conical flask containing
50 ml of sterilized nutrient broth (NB). After incubation for 18 h at
37\2C, the conical flask was removed from the incubator and preserved
in a refrigerator as a stock solution. The concentration of stock solution
was determined by pour plate counts (PPC) method.
Composition of Media
The composition of acetamide broth is acetamide (2.00 g=L), monopotassium
phosphate (0.4 g=L), sodium chloride (0.2 g=L), magnesium sulfate
(0.1 g=L), sodium molibdate (0.05 g=L), and iron sulfate (0.005 g=L);
pH is 7.2. The broth was autoclaved at 121\2C for 15 min.
The composition of acetamide agar is acetamide (10 g=L), sodium
chloride (5 g=L), dipotassium phosphate (1.39 g=L), monopotassium phosphate
(0.73 g=L), magnesium sulfate (0.50 g=L), phenol red (0.012 g=L),
and agar (20 g=L); pH is 7.2. The medium was autoclaved at 121\2C for
15 min.
60 F. He et al.
Downloaded By: [CAS Consortium] At: 03:09 20 April 2009
Apparatus
MSPQC system (Fig. 1a) consists of eight culture bottles (Fig. 1b) and
eight oscillator circuits, shared universal frequency counter, computer,
and temperature control system. The details of the instrument have been
previously reported (He et al. 2006).
MSPQC Method
Culture bottles were placed into MSPQC system and incubated at 37\2C.
Each culture bottle contained 9ml of acetamide broth and 1ml of
P. aeruginosa suspension. The online curves of frequency shift (DF) vs.
culture time could be seen on screen by using self-developed software.

化学论文翻译Until now, no article reported the identification and quantification ofP. aeruginosa using MSPQC.In this article, a new acetamide broth was developed for identificationand quantification of P. aeruginosa by MSPQC. It was based on th
到现在为止,没有文章报道了识别和量化
绿脓杆菌使用MSPQC.
在这篇文章中,一个新的乙酰胺液是专为识别
铜绿假单胞菌和量化的MSPQC.这是基于以下事实：
绿脓杆菌是一个成长中以及在用氮酰胺
和碳源（Noel和霍尔特1984年;史密斯和代顿1972年）.很
一些细菌能够做到这一点.在成长过程中铜绿假单胞菌,
介质电导率的变化,并自动监测
由MSPQC.频率检测时间（FDT的）,它是随着时间的定义在
其中振荡MSPQC频移变快,具有线性
关系,随着浓度的对数.这是一个参数
定量确定由MSPQC传感器绿脓杆菌.
实验
细菌的分离
绿脓杆菌菌（ATCC 9027）,金黄色葡萄球菌（ATCC
12600）,鲍曼不动杆菌菌（ATCC 19606）,大肠埃希氏菌菌（ATCC
11775）和嗜麦芽假单胞菌菌（ATCC 13637）,得到
从湘雅三医院中南大学,中国.
所有的种,接种于血平板购买：（
广东Huankai微生物科技有限公司）在
37 ° C下18小时.然后,每3种纯循环
文化被转移到100毫升含锥形瓶消毒
50毫升灭菌营养肉汤（注）.孵化后18小时在
37摄氏度,锥形烧瓶从孵化器中删除和保存
在作为储备液冰箱.溶液浓度的股票
测定倒板计数（PPC）的方法.
媒体的组成
对乙酰胺液成分是乙酰胺（2.00克= L）的,monopotassium
磷酸盐（0.4克= 1）,氯化钠（0.2克= 1）,硫酸镁
（0.1克= 1）,钠molibdate（0.05克= L）和硫酸亚铁（0.005克= L）的;
pH值是7.2.蒸压肉汤是在121摄氏度15分钟.
对乙酰胺乙酰胺琼脂组成（10克= 1）,钠
氯化铵（5克= 1）,磷酸二氢钾（1.39克= L）和磷酸二氢钾
（0.73克= L）和硫酸镁（0.50克= L）的苯酚红（0.012克= L）的,
和琼脂（20克= 1）,pH值是7.2.该培养基灭菌在121摄氏度的
15分钟.
他60楼等.
下载方式：[中科院在联盟]：2009年4月20日03:09
仪器
MSPQC系统（图1A）的8个文化瓶（图1B）及组成
8振荡器电路,共享通用频率计数器,电脑,
和温度控制系统.该文书的详情,已
以前报告（他等人.2006年）.
MSPQC方法
培养瓶放入MSPQC系统,于37孵育?长
每一种文化中的乙酰胺液瓶和1毫升的九毫升
绿脓杆菌停牌.线上曲线的频移（东风）与
培养时间在屏幕上可以看到通过使用自行开发的软件.