英语翻译In a typical plasmid transformation experiment there will be an extremely heterogeneous mixture of DNA molecules with only a small number consisting of a single vector molecule linked to a single DNA fragment.When E.coli is used for trans

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英语翻译In a typical plasmid transformation experiment there will be an extremely heterogeneous mixture of DNA molecules with only a small number consisting of a single vector molecule linked to a single DNA fragment.When E.coli is used for trans
英语翻译
In a typical plasmid transformation experiment there will be an extremely heterogeneous mixture of DNA molecules with only a small number consisting of a single vector molecule linked to a single DNA fragment.When E.coli is used for transformation the DNA molecules are mixed with the bacterial cells that have been made competent or permeable by being exposed to a cold solution of calcium chloride (0.1M).The cells are then warmed to 37℃ for 5 minutes to allow the DNA to be taken up.The cells are then transferred to broth and after a short incubation period are able to express the functions of the newly acquired chimeric DNA.The bacteria carrying hybrid molecules are identified by a specific screening procedure and purified:their hybrid plasmids are isolated and characterized,or the new products are isolated and studied.Transformation efficiencies are about 10^6 transformants per μg cloning mixture DNA.

英语翻译In a typical plasmid transformation experiment there will be an extremely heterogeneous mixture of DNA molecules with only a small number consisting of a single vector molecule linked to a single DNA fragment.When E.coli is used for trans
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一个典型的质粒转化试验将只有一小的一个向量与单个DNA分子组成的fragment.When大肠杆菌数量的DNA分子极为异构混合物用于改造的DNA分子与细菌混合细胞已取得的资格或渗透，让他们接触到的氯化钙（0.1M的）冷解决方案。细胞，然后加热5分钟，37℃，使脱氧核糖核酸采取up.The细胞，然后转移到肉汤经过短暂的潜伏期能反映新获得的嵌合基因的功能。携带的细菌是由一个特定的筛选程序，确定了混合分...

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一个典型的质粒转化试验将只有一小的一个向量与单个DNA分子组成的fragment.When大肠杆菌数量的DNA分子极为异构混合物用于改造的DNA分子与细菌混合细胞已取得的资格或渗透，让他们接触到的氯化钙（0.1M的）冷解决方案。细胞，然后加热5分钟，37℃，使脱氧核糖核酸采取up.The细胞，然后转移到肉汤经过短暂的潜伏期能反映新获得的嵌合基因的功能。携带的细菌是由一个特定的筛选程序，确定了混合分子和纯化：他们的混合质粒是孤立的特点，或新产品是孤立体系.转化效率约为10 ^ 6次微克克隆转化

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