Does the PCR reaction volume (negatively) influence the outcome?

来源：学生作业帮助网编辑：作业帮时间：2024/11/15 19:37:40

Does the PCR reaction volume (negatively) influence the outcome?
Does the PCR reaction volume (negatively) influence the outcome?

Does the PCR reaction volume (negatively) influence the outcome?
博凌科为-为你No,especially since the introduction of the small,thin walled,0.2 ml plastic vials fitting the 96 well metal blocks of the thermocyclers.A number of observations are worth mentioning:if using older model thermocyclers (without a heated lid),to run small volume PCR reactions,mineral oil is necessary to cover the reaction mixture.Use of oil increases the volume of liquid in the vial and thus can influence somewhat the outcome.Besides,older thermocyclers require the use of larger plastic vials,with thicker walls,that fit less well in the metal blocks and thus may increase the likelihood of variation in PCr outcome.small,thin-walled plastic vials designed for the 96 well metal blocks are ideal for running small-volume PCR reactions.Due to the heated lid of the thermocyclers,there are no mineral oil requirements.When tested,reactions yielded similar results,whether the reaction volume was 100,25 or 5 microliters.It is important to mention,that small volume PCRs may be very beneficial when using small amounts of DNA template.In general,at a constant amount of template DNA,the yield of PCR product per microliter reaction is higher when the reaction volume is 5 L compared to 100 L.This may allow visualisation of the PCR products,sometimes invisible when larger reaction volumes are used.

Does the PCR reaction volume (negatively) influence the outcome? 一道GRE填空题~The ideas expressed in the art historian's book are more ____ than one would expect or the basis of her rather ___ treatment of her subject in the opening pages.A.compelling...intriguingB.accessible...reconditeC.hidebound...reactio when does the chass what does the sign the knod does not The future does not Does Linda write the 热学ΔE,ΔH,q,w,ΔU,ΔE,ΔH,q,w,ΔU,怎么好像ΔE,ΔU都是内能的意思.有什么区别?which of the following statement is CORRECTa.if a reaction occurs at constant pressure,w=ΔEb.if a reaction occurs at constant pressure,q=ΔHc.if a reactio does...matter?原句是：does the preference matter? Does the man help the Does the man know the 谁做过PCR?We carried out an anchored RT-PCR and cloned the low levels of Foxp3 mRNA in the breast tissues.anchored RT-PCR How does the mouse 和 How does the mouse feel When does the unistiy begin. What does the man do? How does the oranges taste? what does the man mean what does the man want