转录组测序中会有one read pair ,two read pairs .read pair 是什么东西啊?

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转录组测序中会有one read pair ,two read pairs .read pair 是什么东西啊?
转录组测序中会有one read pair ,two read pairs .read pair 是什么东西啊?

转录组测序中会有one read pair ,two read pairs .read pair 是什么东西啊?
看你的问题,估计是用illumina的高通量测序,进行RNA-seq的研究吧.
这个问题其实比较简单：
现在的高通量测序,一般分为单端测序（single-end read）和双端测序（paired-end reads）.
单端测序只测模板DNA的一端,一般有36SE、50SE两种.
而双端测序需要讲模板的两端（3‘ 和 5’）都测序,一般有90PE,100PE两种,你提的问题应该是指：read-pair one 和 read-pair tow,是一个模板的两端序列.
注意,paired-end reads中间的是插入片段,并没有进行测序,但是插入片段长度是有意义的.

测序时每次测得高质量片断只有500-600个碱基左右，一次测量称为一个read，将测得的序列进行overlap匹配，称为read pair。

转录组测序中会有one read pair ,two read pairs .read pair 是什么东西啊? read them one by read one's read one‘s Read and circle the different one是什么意思 please read Lesson One.改为否定句 read and circle the odd one Read these words——（one） Your message was read by some one. Peter is one of the____(read)of . for,they,one,only ,$5 ,pai,r are连词成句 基因转录转录哪条链? ()book is she going to read?The new one More than one student has read the book.翻译 twilight is one of（ )books i have read please read after me：“（）one”空里写什么? you should read at______(little)one book a month Read these n____ ,please.Yes,one,two…