英语翻译Effect of Lactobacilli on E.coli Adhesion to Caco-2 Cells in VitroABSTRACT.Inhibitory effect of various lactobacilli against pathogenic strains of E.coli in model system Caco2 cells was determined by enumerating the number of adhering E.c

英语翻译Effect of Lactobacilli on E.coli Adhesion to Caco-2 Cells in VitroABSTRACT.Inhibitory effect of various lactobacilli against pathogenic strains of E.coli in model system Caco2 cells was determined by enumerating the number of adhering E.c
英语翻译
Effect of Lactobacilli on E.coli Adhesion to Caco-2 Cells in Vitro
ABSTRACT.Inhibitory effect of various lactobacilli against pathogenic strains of E.coli in model system Caco2 cells was determined by enumerating the number of adhering E.coli after pre-incubation (exclusion),post-incubation (displacement) or co-incubation (competition) with lactobacilli.Porcine E.coli strain F107(F18ab,Stx2v) in the competition assay with porcine lactobacillus strain P10 gave bacterial counts 7.25 (log CFU per well); in the exclusion test it was only 7.05 while in displacement test it reached 7.29.The lowest E.coli counts adhering to Caco-2 cells were in exclusion assay (pre-incubation,Lactobacillus inoculated as the first).Pre-treatment of E.coli with ou r lactobacilli strains reduced the cultivable E.coli numbers.
Pathogenic strains of E.coli are a major cause of bacterial diarrhea in humans and animals (Vu-Khac et al.2004; Holoda et al.2004).Lactobacilli and other commensal bacteria (e.g.,some bifidobacteria,enterobacteria) provide the protection of the host against possible colonization by the pathogenic bacteria (Reid et al.1998; Mego et al.2005) by inhibiting the pathogen–cell association and invasion (Chauviere et al.1992; Coconier et al.1992; Buj\4áková et al.2004).Lactobacilli are believed to interfere with pathogens by different mechanisms,e.g.,by the production of antimicrobial compounds,such as lactic acid,dihydrogen peroxide,or bacteriocin-like substances (Mc-Croarty et al.1988); some of the Lactobacillus strains coaggregate with pathogenic bacteria (Kme\5 et al.1999; Buj\4áková and Kme\5 2002; Buj\4áková et al.2004).
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英语翻译Effect of Lactobacilli on E.coli Adhesion to Caco-2 Cells in VitroABSTRACT.Inhibitory effect of various lactobacilli against pathogenic strains of E.coli in model system Caco2 cells was determined by enumerating the number of adhering E.c
乳酸菌对试管中大肠杆菌黏着caco-2细胞作用的影响
摘要：在caco-2细胞模型系统中,我们可以通过计数预培养（排除）,过培养（取代）和共培养（竞争）过程中黏着在乳酸菌表面的大肠杆菌的数目来确定乳酸菌对致病大肠杆菌的抑制作用.猪的大肠杆菌F107种与猪的乳酸菌P10种结合体在竞争体系中共用7.25个,在排除体系中只统计到了7.05个,而在取代体系中去发现了7.29个.黏着到caco-2上的大肠杆菌最少的是排除体系（预培养,先接种乳酸菌）.而提前用乳酸菌对大肠杆菌进行处理会减少杂合大肠杆菌的数目.
大肠杆菌的致病种是引起人类和动物细菌性腹泻的主要菌种.乳酸菌和一些其他的共生菌（如,双歧杆菌和肠道菌）为寄主对致病菌的入侵提供了很好的保护作用.这种保护作用是阻止病原细菌对寄主的附着与入侵实现的.人们认为乳酸菌可以通过多种机制对致病菌起抑制作用.比如,通过分泌抗菌化合物如乳酸,二氢过氧化物,或产生类细菌素物质.一些乳酸菌与致病菌coaggregate（这个词不知道啥意思）
粗略翻译,仅供参考,但绝对是手工.