英语翻译Expression of hormone receptors and cell kinetic-related moleculesduring hormone therapy.In initial biopsy specimens,diffuselystrong immunoreactivity for ER and PR and a heterogeneous orsporadic distribution of immunopositive cells for Ki

英语翻译Expression of hormone receptors and cell kinetic-related moleculesduring hormone therapy.In initial biopsy specimens,diffuselystrong immunoreactivity for ER and PR and a heterogeneous orsporadic distribution of immunopositive cells for Ki
英语翻译
Expression of hormone receptors and cell kinetic-related molecules
during hormone therapy.In initial biopsy specimens,diffusely
strong immunoreactivity for ER and PR and a heterogeneous or
sporadic distribution of immunopositive cells for Ki-67,p21
and p27 were found for most tumors.During hormone therapy,
reduction or loss of PR and Ki-67 immunoreactivity was evident
in endometrial carcinomas,in contrast to increased p27
and no alteration in ER or p21 immunoreactivity (Fig.1).There
were significant associations of TE grading with PR,Ki-67,
and p27 LIs (Fig.5).Similar findings were also noted in atypical
hyperplasias (data not shown).
Correlations among β-catenin,hormone receptors and cell kinetic-related
molecules during hormone therapy.As shown in Table 4,the
N-LI values for β-catenin,as well as the gene mutation status
(data not shown),did not correlate with any of the other parameters
investigated,with the exception of the hormone receptor
status in the atypical hyperplasia category.
PR,but not ER,LI values were positively correlated with Ki-
67 and inversely with p27 LIs in both carcinomas and atypical
hyperplasias.Significant correlations for LIs between Ki-67
and either p21 or p27 were also noted.
Effects of progesterone on endometrial carcinoma cell lines.PCRdirect
sequence assays demonstrated an identical exon 3 mutation
of the β-catenin gene with mRNA and genomic DNA samples
from HEC265,featuring a GAC to GTC (Asp to Val)change at codon 32,in contrast to no mutation in Ishikawa
(data not shown).Immunofluorescence findings also showed
more frequent nuclear β-catenin stainings in HEC265,but not
Ishikawa (Fig.6A).
Upon MPA treatment,no apparent morphological changes
were observed in HEC265 and Ishikawa under phase-contrast
microscopy.Western blot assays revealed that lysates obtained
from total cell or membrane/nuclear fractions did not exhibit
any significant changes in β-catenin levels (data not shown).
The cytoplasmic protein level,however,was increased significantly
during MPA treatment in HEC265,together with downregulation
of PR,while there was no apparent change in GSK-
3β expression.In Ishikawa,PR expression levels were also suppressed
by MPA treatment,but the effect was relatively minor
(Fig.6B).No apparent induction of nuclear β-catenin accumulation
was evident in HEC265 and Ishikawa cells (data not
shown).In addition,β-catenin transcriptional functions as assessed
with TCF-responsive luciferase reporter constructs were
not observed in either cell line before or after MPA treatment
(data not shown).

英语翻译Expression of hormone receptors and cell kinetic-related moleculesduring hormone therapy.In initial biopsy specimens,diffuselystrong immunoreactivity for ER and PR and a heterogeneous orsporadic distribution of immunopositive cells for Ki
好长,非医学专业的来译就累人,我先译两段,过两天再译另一半...
激素治疗中激素受体和细胞动力相关分子的表达.在大部分肿瘤的最初活检标本内,发现ER和PR以及一种异质或散发性的Ki-67,p21,p27免疫阳性细胞有非常强烈的免疫反应性.在激素治疗的过程中,PR和Ki-67免疫反应性的减少或缺失对于子宫内膜癌是显著的,相反的,p27增加了,ER或p21的免疫反应则没有变化（图1）.TE的等级与PR、Ki-67、p27 Lis（图5）之间有显著的关联.在非典型增生例子中也有类似发现（数据未显示）.
在激素治疗中,β-catenin、激素受体和细胞动力相关分子的相关性.如表4所示,β-catenin 的N-LI值,以及基因转化情况（数据未显示）,除非典型增生类别的激素受体外,与所有观测到的参数都不相关.
PR值,并非ER,LI值,在癌科和非典型增生例子中,与Ki- 67呈正相关,与p27 Lis呈负相关.Lis 的显著相关性也在Ki-67与p21或Ki-67与p27之间被发现.