英语翻译The thermodynamics of metals ions binding to proteins and other biological molecules can be measured with isothermal titration calorimetry ,which quantifies the binding enthalpy (∆H.) and generates a binding isotherm.A fit of the is

英语翻译The thermodynamics of metals ions binding to proteins and other biological molecules can be measured with isothermal titration calorimetry ,which quantifies the binding enthalpy (∆H.) and generates a binding isotherm.A fit of the is
英语翻译
The thermodynamics of metals ions binding to proteins and other biological molecules can be measured with isothermal titration calorimetry ,which quantifies the binding enthalpy (∆H.) and generates a binding isotherm.A fit of the isotherm provides the binding constant （K）,thereby allowing the free energy (∆G.) and ultimately the entropy (∆S.) of binding to be determined.The temperature dependence of ∆H.can then provide the change in heat capacity (∆CP.) upon binding.However,ITC measurements of metal binding can be compromised by undesired reactions (e.g.,precipitation,hydrolysis,and redox),and generally involve competing equilibria with the buffer and protons,which contribute to the experimental values (KITC,∆HITC).Guidelines and factors that need to be considered for ITC measurements involving metal ions are outlined.A general analysis of the experimental ITC values that accounts for the contributions of metal–buffer speciation and proton competition and provides condition-independent thermodynamic values (K,∆H.) for metal binding is developed and validated.

英语翻译The thermodynamics of metals ions binding to proteins and other biological molecules can be measured with isothermal titration calorimetry ,which quantifies the binding enthalpy (∆H.) and generates a binding isotherm.A fit of the is
金属离子的热力学绑定到蛋白质和其它生物分子可以测定等温滴定热,将约束焓(∆h)和一个有约束力的等温产生.提供一个合适的等温的结合常数(K),因此允许自由能量(∆g)和最终的熵(∆s .)的结合是确定的.h .∆的温度变化关系的改变就能提供热容(∆CP)在约束力.然而,国贸金属结合的测量可以遭受意外的反应(如沉淀法、水解、氧化还原),通常包括竞争的平衡与缓冲和质子,有助于实验值(KITC,HITC∆).指南和因素需要被考虑为国贸测量涉及金属离子和展望.一个大概的分析实验国贸值的贡献占metal-buffer形态与质子的竞争和提供condition-independent热力学值(钾、∆h)对金属结合开发和验证.

要手动麽？