英语翻译In order to obviate the drawbacks of plasma immunoglobulins,the whole molecular recombinant human anti一HAV(hepatitis A virus) monoclonal antibody(anti—HAV IgG) produced and secreted by rCHO cells was purified and its physicochemical p

英语翻译In order to obviate the drawbacks of plasma immunoglobulins,the whole molecular recombinant human anti一HAV(hepatitis A virus) monoclonal antibody(anti—HAV IgG) produced and secreted by rCHO cells was purified and its physicochemical p
英语翻译
In order to obviate the drawbacks of plasma immunoglobulins,the whole molecular recombinant human anti一HAV(hepatitis A virus) monoclonal antibody(anti—HAV IgG) produced and secreted by rCHO cells was purified and its physicochemical properties were extensively characterized． The rCHO cells were cultured in serum-free medium and the superuatants were collected．The recombinant human IgG molecules were sequentially purified by ultrafiltration,rProtein A Sepharose Fast Flow affinity chromatography,ion exchange chromatography and diafiltration．In affinity chromatography,prior to the larget protein elution,an intermediate high salt wash step was inserted,difierent pH and salt concentrations were evaluated for the capacity of removing host cell DNA．The yield of the downstream purification process was approximately 40％.The purity of anti—HAV IgG thus generated was assayed with SEC—HPLC method,integration result showed that the monomeric IgG content was more than 99％ ．Western—blot
was carried out with AP—antiHuman IgG (Fab specific)and AP—antiHuman IgG (Fc specific)respectively．the blot result demonstrated that the anti—HAV IgG is human antibody with Fab and Fc structure ．The specific anti—HAV activity determined by ELISA was l00 IU／mg,with anti—HAV immunoglobulin as the working standard reference．Ligand leakage in the eluate of the affinity column was approximately 32 ng／mg IgG,while after further purification
steps,it was decreased to less than 2 ng／mg IgG．Residual host cell DNA was monitored with solid dot blot assay.DNA can be removed efectively with intermediate high salt wash step in the affinity chromatography．Free sulfhydryl content of anti—HAV IgG was assayed with fluorescent spectmphotometer,the low molecular weight bands appeared in non—reducing SDS-PAGE may be caused by the presence of free sulfhydry1．The endotoxin content was less than 1EU／mg examined by standard LAL test procedures． Anti—HAV IgG prepared with this process is able to fulfill the regulatory requirements of State Food and Drug Administration for recombinant products．
专有名词较多,谢绝软件翻译
失误,贴错了!
是下面这个!
失误,贴错了!
是下面这个!
失误,贴错了!
是下面这个!
1．1 Cell culture with serum free medium
The recombinant cell line secreting anti—HAV was provided by professor Liang Mifang,Chinese Center for Disease Control and Prevention．The anti—HAV is a humanderived mAb of IgG1 subtype． The medium used for cell propagation is DMEM (GIBCO)containing 10％ FBS and 5 x 10 ^-7 mol／L Methotrexate(MTX),the serum free medium used for production is CCM一5 (Hyclone)．
Cells from the cell bank were propagated in a seriesof T—flasks and finally inoculated into roller bottles,roller bottles and roller bottle apparatus were obtained from BELLCO Corporation．After inoculation． cells were cultured in DMEM for 5 to 7 days,and then the mediumwas chan ged to seem free and supernatants were collected and replaced every other day．
软件翻得吧,错误百出啊

英语翻译In order to obviate the drawbacks of plasma immunoglobulins,the whole molecular recombinant human anti一HAV(hepatitis A virus) monoclonal antibody(anti—HAV IgG) produced and secreted by rCHO cells was purified and its physicochemical p
1.1 无血清培养基细胞培养
分泌HAV抗体的重组细胞系由中国疾病预防控制中心的Liang Mifang教授提供.
HAV抗体是一种人类衍生的IgG1免疫蛋白单克隆抗体.培养基是应用DMEM(GIBCO)包含10%的FBS和5 x 107 mol／L氨甲叶酸(MTX), 用于生产的无血清培养基是CCM一5 (Hyclone).
细胞由细胞库中提取后首先在一系列T型瓶中培养,之后接种到BELLCO公司生产的转动瓶培养.接种之后,细胞在DMEM中培养5至7天,之后培养基改变以保持新鲜并且每天收集并更换上清液.
PS:有些东西没怎么接触过所以翻译的可能不准确,比如最后一句那个free